Journal
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
Volume 11, Issue 4, Pages 291-296Publisher
KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
DOI: 10.1007/s13770-014-0046-3
Keywords
Grooved microfiber; Round channel; surface tension; PDMS droplet; neuronal cell guiding
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Funding
- National Research Foundation of Korea (NRF), Republic of Korea [2013046403, 2012R1A6A3A03039473]
- National Research Foundation of Korea [2012R1A6A3A03039473] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Microfibers with groove structure enable the guided culture of diverse cells. For the fabrication of grooved microfibers, microchannel with a few microscale groove structure needs to be fabricated using complicated photolithography processes. In this study, a novel method to fabricate a round channel embedded microfluidic device for generating continuous grooved microfibers is introduced. The surface tension of PDMS prepolymer was used to construct the round channels and groove patterned structures in the microfluidic device. The self-organized multi-droplet structures were utilized to engrave the groove on the microfiber without any complex procedure. Using the device, the groove patterned (ca. 10 mu m of pattern size) microfiber was successfully fabricated. Microfibers were continuously produced without clogging and the diameters of the grooved microfibers were varied with the flow rate. For the test of usability for cell alignment, fibers were coated with laminin and the neuro-progenitor cells from prenatal rat were seeded on the prepared grooved fibers and cultured. The guided growth of neuronal cells on the fiber was observed through the immunostaining with neurofilament.
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