Journal
TISSUE & CELL
Volume 41, Issue 6, Pages 448-455Publisher
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.tice.2009.05.004
Keywords
Mesenchymal stem cell (MSCs); Human; Lung; Sperm
Categories
Funding
- National Natural Science Foundation of China [30200137]
- Key Program of State Education Ministry [109148]
- The Scientific Research Program of Shaanxi Province [2008KO2-05]
- China Postdoctoral Science Foundation funded project [20080431253, 200801438]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry [14110101]
- The Youth Research Key Member Program of Northwest A F University [01140301]
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Pluripotent mesenchymal stem-like cell lines were established from lungs of 3-4 months old aborted fetus. The cells present the high ex vivo expansion potential of MSC, a typical fibroblast-like morphology and proliferate up to 15 passages without displaying clear changes in morphology. Immunological localization and flow cytometry analyses showed that these cells are positive for OCT4, c-Kit, CD11, CD29, CD44, telomerase, CD106, CD105, CD166, and SSEA1 weakly expression or negative for SSEA1, SSEA3, SSEA4, CD34, CD105 and CD106. These cells can give rise to the adipogenic as evidenced by accumulation of lipid-rich vacuoles within cells identified by Oil-red 0 when they were induced with 0.5 mM isobutylmethylxanthine, 200 mu M indomethacin, 10(-6) M dxamethasone, and 10 mu g/ml of insulin in high-glucose DMEM. Osteogenic lineage cells were generated in 0.1 mu M dexamethasone, 50 mu g/ml ascorbic acid, 10 mM beta-glycerophosphate, which are shaped as the osteoblastic morphology, expression of alkaline phosphatase (AP), and the formation of a mineralized extracellular matrix identified by Alizarin Red staining. Neural cells are observed when the cultures were induced with 2-mercapometal, which are positive for nestin, NF-100, MBP and GFAR Additionally, embryoid bodies (EBs) and sperm like cells are obtained in vitro differentiation of these lung MSCs induced with 10(-5) M retinoic acid (RA). These results demonstrated that these MSCs are pluripotent and may provide an in vitro model to study germ-cell formation and also as a potential source of sperms for male infertility. (C) 2009 Elsevier Ltd. All rights reserved.
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