Salvianolic acid B suppresses IFN-γ-induced JAK/STAT1 activation in endothelial cells

Introduction: Dysfunction of the endotheliumcontributes to pathological conditions of the arterialwall including atherosclerosis as a result of immunological and/or inflammatory responses. Salvianolic acid B (Sal B), a pure and active compound extracted from the Chinese herb Salvia miltiorrhizae (SM) was characterized for its anti-inflammatory and anti-oxidant properties on vascular system. Methods and Results: Sal B pretreatment significantly inhibited the IFN-gamma-induced phosphorylations of JAK2 (Tyr 1007/1008) and STAT1 (Tyr701 and Ser727). Consistently, IFN-gamma-induced STAT1 downstream targets CXC chemokines' IP-10, Mig, and I-TAC were suppressed by Sal B pretreatment. Sal B inhibited promoter activities of IP-10 and the secretion of IP-10 protein. The monocyte adhesion to IFN-gamma-treated ECs was observed to be reduced after Sal B pretreatment. ECs treated with Sal B alone also increased the expression of PIAS1 and SOCS1 which may also contribute to its inhibitory effect on JAK-STAT1 signaling pathways. Conclusions: The anti-inflammatory properties of Sal B on IFN-gamma-induced JAK-STAT1 activation were demonstrated in the present study which provides a molecular basis for possible therapeutic usage on vascular disorders. (C) 2011 Elsevier Ltd. All rights reserved.