Journal
MOLECULAR BRAIN
Volume 8, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/s13041-015-0105-2
Keywords
alpha 2-adrenoceptor; Dexmedetomidine; Dorsal root ganglion; Pain; Tetrodotoxin-resistant (TTX-R) sodium channel Na(v)1.8; Whole-cell recording
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Funding
- National Natural Science Foundation of China [31421091, 31420103903, 31271183, 81471130, 81200854]
- National Basic Research Program of China [2013CB531900]
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Background: Systemically administered dexmedetomidine (DEX), a selective alpha 2 adrenergic receptor (alpha 2-AR) agonists, produces analgesia and sedation. Peripherally restricted alpha 2-AR antagonist could block the analgesic effect of systemic DEX on neuropathic pain, with no effect on sedation, indicating peripheral analgesic effect of DEX. Tetrodotoxin-resistant (TTX-R) sodium channel Na(v)1.8 play important roles in the conduction of nociceptive sensation. Both a2-AR and Na(v)1.8 are found in small nociceptive DRG neurons. We, therefore, investigated the effects of DEX on the Na(v)1.8 currents in acutely dissociated small-diameter DRG neurons. Results: Whole-cell patch-clamp recordings demonstrated that DEX concentration-dependently suppressed TTX-R Nav1.8 currents in small-diameter lumbar DRG neurons. DEX also shifted the steady-state inactivation curves of Nav1.8 in a hyperpolarizing direction and increased the threshold of action potential and decrease electrical and chemical stimuli-evoked firings in small-diameter DRG neurons. The a2-AR antagonist yohimbine or alpha 2(A)-AR antagonist BRL44408 but not alpha 2(B)-AR antagonist imiloxan blocked the inhibition of Na(v)1.8 currents by DEX. Immunohistochemistry results showed that Na(v)1.8 was predominantly expressed in peripherin-positive small-diameter DRG neurons, and some of them were alpha 2(A)-AR-positive ones. Our electrophysiological recordings also demonstrated that DEX-induced inhibition of Na(v)1.8 currents was prevented by intracellular application of G-protein inhibitor GDP beta-s or G(i/o) proteins inhibitor pertussis toxin (PTX), and bath application of adenylate cyclase (AC) activator forskolin or membrane-permeable cAMP analogue 8-Bromo-cAMP (8-Br-cAMP). PKA inhibitor Rp-cAMP could mimic DEX-induced inhibition of Nav1.8 currents. Conclusions: We established a functional link between alpha 2-AR and Na(v)1.8 in primary sensory neurons utilizing the G(i/o)/AC/cAMP/PKA pathway, which probably mediating peripheral analgesia of DEX.
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