Journal
THIN SOLID FILMS
Volume 519, Issue 3, Pages 1213-1218Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.tsf.2010.08.071
Keywords
Aflatoxin; Cysteamine; Gold nanoparticles; Immunosensor; Self assembled monolayer
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Funding
- National Research Foundation of Korea [R32-2008-000-20026-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Aflatoxin B-1 antibody (aAFB(1)) covalently attached to cysteamine functionalized-gold nanoparticles (C-AuNP) has been immobilized onto 4-mercaptobenzoic acid (MBA) based self assembled monolayer (SAM) on gold electrode (MBA/Au), for the fabrication of BSA/aAFB(1)-C-AuNP/MBA/Au immunoelectrode. This immunoelectrode has been characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) and electrochemical characterization techniques. The electrochemical response studies reveal that the BSA/aAFB(1)-C-AuNP/MBA/Au immunoelectrode can be used to detect AFBI in the range of 10-100 ng dL(-1) and has sensitivity as 0.45 mu A ng(-1)dL, limit of detection as 17.90 ng dL(-1) and a response time of 60 s. (C) 2010 Elsevier B.V. All rights reserved.
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