Journal
THIN SOLID FILMS
Volume 518, Issue 17, Pages 5010-5013Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.tsf.2010.03.077
Keywords
Gold nanoparticles; Self-assembled monolayers; Piezoelectric immunosensor; Carcinoembryonic antigen
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Funding
- Education Ministry Projects of Hunan Province [09C700]
- Natural Science Foundation of Hunan Province [07JJ3015]
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It is very important for a piezoelectric immunosensor to increase specific binding and decrease nonspecific adsorption. This study presents the development of such a piezoelectric immunosensor for the detection of carcinoembryonic antigen. An AT-cut quartz crystal's Au electrode surface was first modified with homogenous self-assembled monolayer of cysteamine (CE). Gold nanoparticles capped with mixed self-assembled monolayer of CE and MH (6-mercapto-1-haxanol) were then attached to the CE monolayer via glutaraldehyde (GA). Antibodies were immobilized onto a mixed self-assembled monolayer of CE and MH with GA as a reactive intermediate too. The binding of target antigens onto the immobilized antibodies decreased the sensor's resonant frequency, and the frequency shift was correlated to the antigen concentration. The stepwise assembly of the immunosensor was characterized by means of cyclic voltammetry technique. This immunoassay was shown to be specific and sensitive, thus providing a viable alternative to carcinoembryonic antigen detection method. (C) 2010 Elsevier B.V. All rights reserved.
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