Journal
THIN SOLID FILMS
Volume 519, Issue 3, Pages 1196-1201Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.tsf.2010.08.069
Keywords
Zinc oxide (ZnO); DNA biosensor; Electrochemical deposition
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Nanostructured zinc oxide (nsZnO) films have been fabricated onto conducting indium-tin-oxide (ITO) coated glass plate, by cathodic electro-deposition to immobilize probe DNA specific to M. tuberculosis via physisorption based on strong electrostatic interactions between positively charged ZnO (isoelectric point = 9.5) and negatively charged DNA to detect its complementary target. Electrochemical studies reveal that the presence of nano-structured ZnO results in increased electro-active surface area for loading of DNA molecules. The DNA-nsZnO/ITO bioelectrode exhibits interesting characteristics such as detection range of 1 x 10(-6) - 1 x 10(-12) M, detection limit of 1 x 10(-12) M (complementary target) and 1 x 10(-13) M (genomic DNA), reusability of about 10 times, response time of 60s and stability of up to 4 months when kept at 4 degrees C. (C) 2010 Elsevier B.V. All rights reserved.
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