Journal
SCIENTIFIC WORLD JOURNAL
Volume -, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2014/141675
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Funding
- National Natural Science Foundation of China [41171216]
- One Hundred Talent Plan of CAS
- CAS/SAFEA International Partnership Program for Creative Research Teams
- Yantai Science & Technology Development Project [2011016]
- Yantai Double-Hundred Talent Plan [XY-003-02]
- 135 Development Plan of YIC-CAS
- Science & Technology Development Plan of Shandong Province [010GSF10208]
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A new plasma membrane Na+/H+ antiporter gene (named as KvSOS1) was cloned from the halophyte Kosteletzkya virginica by reverse-transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technology, which is a homologue of SOS1 (salt overly sensitive 1). The full-length cDNA is 3850 bp and contains an open reading frame (ORF) encoding a protein of 1147 amino acids with a molecular weight of 127.56 kDa and a theoretical pI of 6.18. Bioinformatics analysis indicated that the deduced protein appears to be a transmembrane protein with 12 transmembrane domains at the N-terminal region and a long hydrophilic tail in cytoplasm at its C-terminal region and shares 72-82% identity at the peptide level with other plant plasma membrane Na+/H+ antiporters.
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