Journal
THERIOGENOLOGY
Volume 78, Issue 4, Pages 747-752Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2012.03.021
Keywords
Bovine; Individual culture; Oxidative stress; Sericin
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The silk protein sericin has been identified as a potent antioxidant in mammalian cells. This study was conducted to examine the effects of sericin on preimplantation development and quality of bovine embryos cultured individually. When two-cell-stage embryos were cultured individually for 7 days in CR1aa medium supplemented with 0, 0.1, 0.5, or 1% sericin, rates of total blastocyst formation and development to expanded blastocysts from embryos cultured with 0.5% sericin were higher (P < 0.05) than those from embryos cultured with 0 or 1% sericin. When embryos were cultured individually for 7 days in the CR1aa medium supplemented with 0 or 0.5% sericin under two oxidative stress conditions (50 or 100 mu M H2O2), the addition of sericin significantly improved the blastocyst formation rate of embryos exposed to 100 mu M H2O2. However, the protective effect of sericin was not observed in development of embryos exposed to 50 mu M H2O2. When embryos were exposed to 100 mu M H2O2 during culture, the DNA fragmentation index of total blastocysts from embryos cultured with 0.5% sericin was lower than blastocysts derived from embryos cultured without sericin (4.4 vs. 6.8%; P < 0.01). In conclusion, the addition of 0.5% sericin to in vitro culture medium improved preimplantation development and quality of bovine embryos cultured individually by preventing oxidative stress. (C) 2012 Elsevier Inc. All rights reserved.
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