Journal
THERIOGENOLOGY
Volume 74, Issue 3, Pages 458-465Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2010.02.029
Keywords
Sperm; Stallion; Mitochondria; Sublethal damage; Apoptosis; Bongkrekic acid; Cryopreservation
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Funding
- Ministerio de Educacion y Ciencia, FEDER Madrid, Spain [AGL 2007-60598 (GAN), BFU-2007-62423 (BFI), INIA RZ2008-00018-00-00]
- Swedish Foundation for Equine Research (SSH), Stockholm, Sweden
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In order to evaluate to what extent the changes that occur during cryopreservation involve the mitochondrial permeability transition pore (PT-pore), a specific inhibitor was used during the cryopreservation process of stallion spermatozoa. Four ejaculates from each of 7 stallions were frozen using a standard protocol. Before freezing, each ejaculate was split into three subsamples. The first was supplemented with 2.5 mu M bongkrekic acid (BA) and the second with 5 mu M BA. The third subsample served as control. The BA significantly reduced the percentage of spermatozoa depicting active caspases after thawing, reduced the percentage of spermatozoa with increased membrane permeability, and increased the mitochondrial membrane potential of thawed sperm. Sperm motility was reduced as a result of the treatment. It is concluded that the mitochondrial pathway of apoptosis seems to be an important factor involved in the sublethal damage that equine spermatozoa experience after freezing and thawing, and that sperm motility in the equine species is largely dependent on mitochondrial ATP produced by oxidative phosphorylation. (C) 2010 Elsevier Inc. All rights reserved.
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