Journal
THEORETICAL AND APPLIED GENETICS
Volume 126, Issue 12, Pages 3009-3020Publisher
SPRINGER
DOI: 10.1007/s00122-013-2189-3
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Funding
- Sino-Swiss Joint Research Project [2009 DFA32040, IZLCZ3 123946I]
- Natural Science Foundation of China [30900887]
- Zhejiang Provincial Innovation Team of Nuclear Agricultural Science and Technology [2010R50033]
- Natural Science Foundation [LY13C130002]
- Special Fund for Agro-scientific Research in the Public Interest [201103007]
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The rice low phytic acid (lpa) mutant Os-lpa-XS110-1(XS-lpa) has similar to 45 % reduction in seed phytic acid (PA) compared with the wild-type cultivar Xiushui 110. Previously, a single recessive gene mutation was shown to be responsible for the lpa phenotype and was mapped to a region of chromosome 3 near OsMIK (LOC_Os03g52760) and OsIPK1 (LOC_Os03g51610), two genes involved in PA biosynthesis. Here, we report the identification of a large insert in the intron of OsMIK in the XS-lpa mutant. Sequencing of fragments amplified through TAIL-PCRs revealed that the insert was a derivative of the LINE retrotransposon gene LOC_Os03g56910. Further analyses revealed the following characteristics of the insert and its impacts: (1) the inserted sequence of LOC_Os03g56910 was split at its third exon and rejoined inversely, with its 5' and 3' flanking sequences inward and the split third exon segments outward; (2) the LOC_Os03g56910 remained in its original locus in XS-lpa, and the insertion probably resulted from homologous recombination repair of a DNA double strand break; (3) while the OsMIK transcripts of XS-lpa and Xiushui 110 were identical, substantial reductions of the transcript abundance (similar to 87 %) and the protein level (similar to 60 %) were observed in XS-lpa, probably due to increased methylation in its promoter region. The above findings are discussed in the context of plant mutagenesis, epigenetics and lpa breeding.
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