Journal
THEORETICAL AND APPLIED GENETICS
Volume 126, Issue 7, Pages 1713-1720Publisher
SPRINGER
DOI: 10.1007/s00122-013-2086-9
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Funding
- DFG [1070/1]
- International Research Training Group Sustainable Resource Use in North China
- Bill & Melinda Gates Foundation
- [CARS-02-09]
- [2009CB118400]
- [2011AA10A103]
- [2012AA10A305]
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Production of haploids by the in vivo haploid induction method has now become routine for generating new inbred lines in maize. In previous studies, a major quantitative trait locus (QTL) (qhir1) located in bin 1.04 was detected, explaining up to 66 % of the genotypic variance for haploid induction rate (HIR). Our objectives were to (1) fine-map qhir1 and (2) identify closely linked markers useful for marker-assisted breeding of new inducers. For this purpose, we screened a mapping population of 14,375 F-2 plants produced from a cross between haploid inducer UH400 and non-inducer line 1680 to identify recombinants. Based on sequence information from the B73 reference genome, markers polymorphic between the two parents were developed to conduct fine mapping with these recombinants. A progeny test mapping strategy was applied to accurately determine the HIR of the 14 recombinants identified. Furthermore, F-3 progeny of recombinant F-2 plants were genotyped and in parallel evaluated for HIR. We corroborated earlier studies in that qhir1 has both a significantly positive effect on HIR but also a strong selective disadvantage, as indicated by significant segregation distortion. Altogether, we were able to narrow down the qhir1 locus to a 243 kb region flanked by markers X291 and X263.
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