Journal
JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY
Volume 2015, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2015/514320
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Funding
- National Nature Science Foundation of China [21105124, 21277174, 21347002]
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A liquid chromatographic tandem mass spectrometry method (LC-MS/MS) for the simultaneous determination of 1-hydroxypyrene (1-OHP), 3-hydroxybenzo[a] pyrene (3-OHBaP), and 3-hydroxybenz[a] anthracene (3-OHBaA) in human urine has been developed. With the exception of 3-OHBaP at a low spiking level, the average recoveries were greater than 80%. The method has good accuracy (72.1-107.7%) and reproducibility (1.8-11.4%) and was successfully used to study the uptake of pyrene, benzo[a] pyrene, and benzo[a] anthracene from cigarette smoke. The results indicated that urinary 1-OHP concentration in the smoking group (66.58 +/- 70.91 ng/g creatinine) was higher than that observed in the nonsmoking group (58.16 +/- 49.48 ng/g creatinine). Urinary 3-OHBaA concentrations in nonsmokers and smokers with 8 mg and 10 mg tar cigarettes were 10.98 +/- 4.39 ng/g creatinine, 11.01 +/- 13.30 ng/g creatinine, and 9.17 +/- 12.89 ng/g creatinine, respectively. Urinary 3-OHBaP concentrations in nonsmokers and smokers with 8mg and 13 mg tar cigarettes were 1.30 +/- 0.20 ng/g creatinine, 2.83 +/- 1.78 ng/g creatinine, and 6.00 +/- 4.44 ng/g creatinine, respectively. Urinary 1-OHP levels exhibited a significant correlation with BaP yield in cigarette smoke under the Canadian intense smoking condition (y = 3.5563x + 30.171, R-2 = 0.9916, n = 227).
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