Journal
TETRAHEDRON LETTERS
Volume 54, Issue 24, Pages 3063-3066Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tetlet.2013.03.136
Keywords
Carbohydrate; 4-Nitrocatechol; AXH-d(3); alpha-L-Arabinofuranosidase
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Funding
- Region Midi-Pyrenees Grant DAER Recherche [10008500]
- Region Midi-Pyrenees
- European Regional Development Fund
- SICOVAL
- Centre National de la Recherche Scientifique (CNRS)
- Institut National de la Recherche Agronomique (INRA)
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The synthesis of a compound containing a 4-nitrocatechol bound to two vicinal alpha-L-arabinofuranosyl moieties through a linker arm was achieved using a sulfate protecting group to facilitate selective alkylation of one aromatic hydroxyl. Several alpha-L-arabinofuranosidases displaying different selectivities were tested and a simple microtiter plate-based assay was developed. The observed resistance of the compound to alpha-L-arabinofuranosidase-mediated hydrolysis makes it suitable for the identification of enzymes that are able to accommodate bis-arabinofuranosylated moieties. (C) 2013 Elsevier Ltd. All rights reserved.
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