Journal
TETRAHEDRON LETTERS
Volume 50, Issue 9, Pages 1082-1085Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tetlet.2008.12.094
Keywords
Aspergillus glaucus; Biosynthesis; Polyketide pathway; C-13 NMR; Feeding experiments; Natural products
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Funding
- National High Technology Research and Development Program of China [2006AA09Z429]
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Aspergiolide A, a novel antitumor compound, was produced by a marine-derived filamentous fungus Aspergillus glaucus. The biosynthesis of it was unambiguously determined by feeding experiments using [1-C-13]sodium acetate, [2-C-13]sodium acetate, and [1,2-C-13(2)]sodium acetate precursors followed by C-13 NMR spectroscopic investigation of the isolated products. Analysis of the patterns of C-13-enrichment revealed that all 25 carbon atoms in skeleton of aspergiolide A were derived from labeled acetate. And among them, 12 carbon atoms were labeled from the carboxylic group of acetate, while the other 13 carbon atoms were labeled from the methylic group of acetate. Besides, the labeling pattern of [1,2-C-13(2)]sodium, acetate feeding experiment demonstrated that 12 intact acetate units were incorporated in aspergiolide A by polyketide pathway. (c) 2008 Elsevier Ltd. All Fights reserved.
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