Journal
TETRAHEDRON
Volume 68, Issue 9, Pages 2141-2145Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tet.2012.01.020
Keywords
Biocatalysis; Solvent effects; Bio-solvents; Transglycosylation; beta-Galactosidase
Categories
Funding
- MICINN ('Ministerio de Ciencia e Innovacion') [CTQ2009-11801]
- Universidad Nacional de Costa Rica
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Bio-solvents are good alternative solvents that avoid the use of classical organic solvents when performing enzymatic reactions. A noticeably change in regioselectivity was observed in the synthetic behaviour of Biolacta beta-galactosidase using bio-solvents derived from dimethylamide and glycerol as co-solvents. Under these conditions, the enzyme changes its well known tendency to produce beta-(1 --> 4) to beta-(1 --> 6) disaccharides. An evaluation of the bio-solvent concentration and the effects of the non proteic additives in commercially available Biolacta beta-galactosidase was undertaken in order to optimize the reaction conditions to improve the yield of the beta-(1 --> 6) product. (C) 2012 Elsevier Ltd. All rights reserved.
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