Journal
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
Volume 7, Issue 2, Pages 109-116Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/153303460800700203
Keywords
plasmids; gene electrotransfer; GFP; muscle; silencing; shRNA
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RNA interference appears as a promising tool for therapeutic gene silencing. A key limit is the delivery of the siRNA. A safe approach is to use a physical method such as in vivo electropulsation with contact electrodes. Getting a long lived silencing can be better approached by using the in situ expression of shRNA. This is presently obtained by using co-electrotransfer of specific plasmids coding for expression and silencing of a fluorescent protein. Using a non invasive fluorescence imaging assay, electrodelivery in mouse muscles is observed to induce complete silencing over more than two months in a specific way. The proper choices of the plasmids (sequence and relative amounts) and of the electric pulsing conditions appear as key parameters in the successful silencing.
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