Journal
TALANTA
Volume 119, Issue -, Pages 538-543Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2013.11.065
Keywords
Graphene quantum dots; Biothiol compound; Fluorescence; Biosensor
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Funding
- 100 Talents program of the Chinese Academy of Sciences
- National Basic Research Program of China (973 Program) [2011CB911002]
- National Natural Science Foundation of China [21075119, 91027036, 21275139]
- Pillar Program of Changchun Municipal Bureau of Science and Technology [2011225]
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A facile assay method for the highly sensitive and selective sensing of biothiols based on graphene quantum dots (GQDs) has been developed. GQDs emitted strong blue fluorescence in an aqueous buffer solution. It was observed that mercury(II) ions could efficiently bind and quench the fluorescence of the GQDs. When a biothiol compound (glutathione, cysteine, or homocysteine) was added to the assay mixture of GQDs and mercury(II), it bound to mercury(II) ions. Hg2+-GQD complex dissociated, and a fluorescence turn-on signal was detected. The emission intensity changes of the GQDs could be directly related to the amount of biothiol added to the assay solution. The assay is highly sensitive, the limits of detection CLOD) for GSH, Cys and Hcy were 5 nM, 2.5 nM and 5 nM, respectively. The assay is also highly selective, a number of amino acids and proteins were tested, and little interference was observed. In addition, GSH standard recovery in serum samples was also demonstrated. We envision that our assay method could facilitate the biothiol quantification related biological and biomedical research. (C) 2013 Elsevier B.V. All rights reserved.
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