Quantum dots-based ratiometric fluorescence probe for mercuric ions in biological fluids

Fluorescence analysis by means of a single fluorescence signal output usually leads to the signal fluctuation caused by various external factors. Ratiometric fluorescence probes that can significantly eliminate the external effects by self-calibration of two different emission bands are preferable for the detection of real samples. In this work, we designed a dual-emission quantum dots (QDs) nanocomposite as a ratiometric probe for the visual detection of Hg2+. The dual-emission QDs nanocomposite consists of two differently sized CdTe/CdS QDs. The red-emitting larger sized CdTe/CdS QDs embeded in silica nanoparticles are insensitive to Hg2+, while the green-emitting smaller sized ones are covalently conjugated onto the silica nanoparticles surface and sensitive to Hg2+. The addition of Hg2+ can only quench green fluorescence in the dual-emission QDs nanocomposites, which triggers the change of fluorescence intensity ratio of two different emission wavelengths and hence induces the evolution of fluorescence color of the probe solution with variation of Hg2+ concentration. Based on this feature, the dual-emission QDs nanocomposites can be used to develop a ratiometric fluorescence probe for the visual detection of Hg2+. Under the optimized conditions, the ratiometric fluorescence QDs probe shows a linear relationship between fluorescence intensity ratio and Hg2+ concentration in the range of 5-300 nM. The detection limit of this probe was found to be 3.1 nM. This ratiometric assay also exhibits a high selectivity and it has been successfully used in the determination of Hg2+ content in fetal bovine serum and human urine. (C) 2013 Elsevier B.V. All rights reserved.