Journal
TALANTA
Volume 122, Issue -, Pages 135-139Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2014.01.016
Keywords
HCPE; ERGO-AuPdNPs; IL-6; AgNPs; Immunosensor
Categories
Funding
- National Basic Research Program of China [2011CB933502]
- National Natural Science Foundation of China [21335004, 21121091]
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A facile one-step electrochemical reduction method was developed to prepare electrochemically reduced graphene oxide (ERGO) and gold-palladium bimetallic nanoparticles (AuPdNPs) as the platform of immunosensor. A novel competitive electrochemical immunosensor was then proposed by combining the ERGO-AuPdNPs platform with silver nanoparticles (AgNPs) functionalized polystyrene bionanolabel for the sensitive detection of human interleukin-6 (IL-6). An electrically heated carbon electrode (HCPE) was introduced in the detection procedure of the immunosensor, and further improved the sensitivity. The immunosensor exhibited a wide linear response to IL-6 ranging from 0.1 to 100000 pg mL(-1) with a detection limit of 0.059 pg mL(-1). The proposed method showed good precision, broad linear range, acceptable stability and high reproducibility, and could be used for the detection of IL-6 in real samples, which possessed promising application in clinical research. (C) 2014 Elsevier B.V. All rights reserved.
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