Journal
TALANTA
Volume 128, Issue -, Pages 305-310Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2014.04.051
Keywords
Detection; Colorimetry; Oligonucleotides; Nanoparticles; Non-negative matrix factorization
Categories
Funding
- National Natural Science Foundation of China (NSFC) [20927003, 90913013, 41273093, 21175101]
- National Major Scientific Instruments and Device Development Project [2012YQ16000701]
- Foundation of China Geological Survey [12120113015200]
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Herein, a one-tube colorimetric platform has been developed for the simultaneous determination of two analytes (DNA as model object) in one tube with picomolar sensitivity. SPR-active nanoparticles are used to encode reporter probes sensitive to oligonucleotides associated with hepatitis A virus Vall7 polyprotein gene (HVA) and hepatitis B virus surface-antigen gene (HVB) respectively and magnetic beads (MBs) serve as the removal tool. In this mixed nanoparticles based biosensor, the addition of target analytes could change the concentration of each nanoparticle, leading to different colors of the supernatant. The influence of spectral overlap has been eliminated by a non-negative matrix factorization (NMF). With the assistance of NMF, the limit of detection (LOD) can be determinated as pM level without amplification. On the whole, this nanosensor boasts the advantages of high sensitivity and low sample consumption. Simultaneous colorimetric detection and quantification of two molecules in one tube are demonstrated. (C) 2014 Elsevier B.V. All rights reserved.
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