Journal
TALANTA
Volume 127, Issue -, Pages 191-195Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2014.04.011
Keywords
Restrict-access materials; Sample extraction; Proteomics; Serum peptides; Two-dimensional
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Funding
- National Natural Science Foundation of China [21105036, 81373374]
- Program for New Century Excellent Talents in University [NCET-12-0234]
- Jilin Provincial Science & Technology Department [20130522057JH]
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The selective extraction of endogenous serum peptides has been a challenge due to the high abundant proteins present in serum. Here a simple on-line extraction of peptides from human serum using strong cation-exchange diol silica restricted-access materials (SCX-RAM) coupled with two-dimensional RP-RP liquid chromatography mass spectrometry was developed. The operation of the on-line extraction system is simple to use and does not need complex equipments. The two-dimensional RP-RP was proved to be orthogonal and efficient to separate peptides extracted from human serum. (C) 2014 Elsevier B.V. All rights reserved.
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