Journal
TALANTA
Volume 120, Issue -, Pages 376-385Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2013.12.004
Keywords
Surface imprinting; Magnetic nanoparticles; Itaconic acid; Bovine hemoglobin
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Funding
- National Natural Science Foundation of China [21175040, 21375035, J1210040]
- Foundation for Innovative Research Groups of NSFC [21221003]
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The protein imprinted technique combining surface imprinting and nano-sized supports materials is an attractive strategy for protein recognition and rapid separation. In this work, we imprinted bovine hemoglobin (BHb) on magnetic nanoparticles. With itaconic acid (IA) and acrylamide (AAm) as the monomers, the experiment was carried out in aqueous media via surface-imprinting technique. The effects of initial concentration and adsorption time over the adsorption capacity of both imprinted and non-imprinted nanoparticles were analyzed. The maximum adsorption capability of imprinted nanoparticles was found to be 77.6 mg g(-1), which was 3.1-4.3 times higher than that of the non-imprinted nanoparticles prepared at the same conditions. This resulted in the successful formation of imprinting cavities. Moreover, in selective adsorption experiment and competitive batch rebinding test, imprinted nanoparticles exhibited a high specific recognition of the template protein over the non-imprinted protein. (C) 2013 Elsevier B.V. All rights reserved.
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