Journal
TALANTA
Volume 104, Issue -, Pages 44-52Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2012.10.088
Keywords
Antioxidant activities; CPE; Marine biotechnology; Phenolic compounds; PLE; SFE; Solid/liquid extraction; Sargassum muticum
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Funding
- Ministere de l'Education Nationale, de l'Enseignement Superieur et de Recherche (UBO allocation)
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A comparative study between alternative extraction processes such as centrifugal partition extraction (CPE), supercritical fluid extraction (SFE) and pressurized liquid extraction (PLE) and classical solid/liquid used in the laboratory are currently focusing on the efficiency (selectivity and productivity) to obtain bioactive phenolic compounds from the phaeophyte Sargassum muticum model. The choice of the best process was based on several measurements: (i) the total phenolic content measured by the colorimetric Folin-Ciocalteu assay, (ii) radical scavenger and antioxidant activities assessed by the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay, and the beta-carotene bleaching method and finally (iii) the method productivity. Irrespective of the solvent used in the processes, alternative methods are always sharply more effective than classical ones. With the exception of SFE which does not allow extracting the totality of the active phenolic compounds, two of the other extraction methods were particularly promising. First, CPE afforded the most important yields in concentrated phenolic compounds (PC) (22.90 +/- 0.65%DW) also displaying the best activities (0.52 +/- 0.02 and 0.58 +/- 0.19 mg/ mL for IC50 and AAC(700), respectively). Secondly, PLE using an EtOH:water mixture 75:25 (v/v) allowed a good PC extraction (10.18 +/- 0.25%DW) with huge efficiency. Despite a lesser activity of the extracts (0.77 +/- 0.01 and 1.59 +/- 0.15 mg/mL for IC50 and AAC(700), respectively) PLE is a green process and potentially complies European norms requirements for the prospective valorization of phenolic compounds from S. muticum in Brittany. (C) 2012 Elsevier B.V. All rights reserved.
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