Journal
TALANTA
Volume 114, Issue -, Pages 304-310Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2013.05.046
Keywords
Scutellaria baicalensis; Scutellariae radix; Baicalin; Baicalein; ATR-IR; NIR
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In this study methods for the quantification of baicalin and total baicalein in Scutellariae radix with near infrared (NIR) spectroscopy and attenuated-total-reflectance mid-infrared (ATR-IR) spectroscopy in hyphenation with multivariate analysis were developed and compared. The reference analysis was performed by high performance liquid chromatography coupled to diode array detection (HPLC-DAD). Different pretreatments like standard normal variate (SNV), multiplicative scatter correction (MSC), first and second derivative Savitzky-Golay were applied on the spectra to optimize the calibrations. A principal component analysis was performed with both spectroscopic methods to distinguish wild and cultivated samples. Quality parameters obtained for test-set calibration models of ATR-IR spectroscopy (baicalin: standard error of prediction (SEP)=1.31, ratio performance to deviation (RPD)=2.91 and R-2=0.88; total baicalein: SEP=1.02, RPD=3.24 and R-2=0.89) and NIR spectroscopy (baicalin: SEP=1.50, RPD=2.54 and R-2=0.88; total baicalein: SEP=1.19, RPD=2.76 and R-2=0.84) demonstrate that both spectroscopic techniques in combination with multivariate analysis are successful tools for the quantification of baicalin and total baicalein in Scutellariae radix, but it was found that ATR-IR spectroscopy provides higher accuracy in the given application. Furthermore it was proved that wild and cultivated samples can be distinguished by ATR-IR. (C) 2013 Elsevier B.V. All rights reserved.
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