Journal
TALANTA
Volume 115, Issue -, Pages 694-701Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2013.06.036
Keywords
Measles-antigen; Measles-specific antibodies; Horse-radish peroxidase; Labeled-detection; Immunosensors
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Funding
- Mintek through Department of Science and Technology (DST)/Mintek Nanotechnology Innovation Centre (NIC)
- National Research Foundation (NRF) through DST/NRF South Africa Research Chairs Initiative for Professor of Medicinal Chemistry and Nanotechnology
- Rhodes University
- NRF South Africa [TTK1207183385]
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The design of electrochemical immunosensors for the detection of measles-specific antibodies is reported. The measles-antigen modified surface was used as an antibody capture surface. The detection of measles-specific IgG antibodies was accomplished using the voltammetric method and horse-radish peroxidase (HRP) labeled secondary antibody (anti-IgG) as a detecting antibody. The potential applications of the designed immunosensor were evaluated in buffer and serum solutions. The immunosensor exhibited good linearity at concentrations less than 100 ng mL(-1) with R-2=0.997 and the limit of detection of 6.60 ng mL(-1) at 3 sigma. The potential application of the immunosensor was evaluated in the deliberately infected human and newborn calf serum samples with measles-IgG antibody mimicking real-life samples. The designed electrochemical immunosensor could differentiate between infected and un-infected serum samples as higher catalytic currents were obtained for infected serum samples. (C) 2013 Elsevier B.V. All rights reserved.
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