Journal
TALANTA
Volume 106, Issue -, Pages 399-407Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2013.01.024
Keywords
Phenolic compound; Enzymatic electrochemical detection; Multivariate calibration; Horseradish peroxidase; Natural water
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Funding
- Universidad Nacional del Litoral, Santa Fe, Argentina [1545, 538501/01]
- Ministry of Science and Innovation (MICINN, Madrid, Spain) [BIO2007-63300, BIO2010-17566]
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An approach based on the electrochemical detection of the horseradish peroxidase enzymatic reaction by means of square wave voltammetry was developed for the determination of phenolic compounds in environmental samples. First, a systematic optimization procedure of three factors involved in the enzymatic reaction was carried out using response surface methodology through a central composite design. Second, the enzymatic electrochemical detection coupled with a multivariate calibration method based in the partial least-squares technique was optimized for the determination of a mixture of five phenolic compounds, i.e. phenol, p-aminophenol, p-chlorophenol, hydroquinone and pyrocatechol. The calibration and validation sets were built and assessed. In the calibration model, the LODs for phenolic compounds oscillated from 0.6 to 1.4 x 10(-6) mol L. Recoveries for prediction samples were higher than 85%. These compounds were analyzed simultaneously in spiked samples and in water samples collected close to tanneries and landfills. Published by Elsevier B.V.
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