Journal
TALANTA
Volume 109, Issue -, Pages 91-100Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2013.01.055
Keywords
Ciprofloxacin; Difloxacin; Lomefloxacin; Norfloxacin; Ofloxacin; Stability; Oxidation studies; Kinetic evaluation; UPLC-MS/MS
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A simple, sensitive and reproducible ultra-performance liquid chromatography method for determination of ciprofloxacin, difloxacin, lomefloxacin, norfloxacin and ofloxacin oxidation stability under permanganate treatment in acidic conditions at pH from 3.0 to 6.0, was developed. Chromatographic separations were carried out using the Acquity UPLC BEH C-18 column; (2.1 x 100 mm, 1.7 mu m particle size). The column was maintained at 40 degrees C, and eluted under isocratic conditions using 83% of eluent A and 17% of eluent B over 6.5 min, at a flow rate of 0.3 mL min(-1). Eluent A: water/formic acid (0.1 v/v%); eluent B: acetonitrile/formic acid (0.1 v/v%). An oxidation process followed kinetic of the second order reaction and depended upon solution acidity. Oxidation of fluoroquinolones proceeded at piperazine moiety yielding respective hydroxy and oxo analogs, and remaining the quinolone fragment intact. Structures of products formed were assigned on a basis of UPLC/MS/MS fragmentation pathways. (C) 2013 Elsevier B.V. All rights reserved.
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