Journal
TALANTA
Volume 104, Issue -, Pages 32-38Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2012.11.014
Keywords
Cardiac troponin I (cTnI); Nanoarray protein chip; High-sensitivity; Total internal reflection fluorescence microscopy (TIRFM)
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Funding
- National Research Foundation (NRF)
- Korean Government (MEST) [2012-0001130]
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2012R1A2A2A01013466]
- National Research Foundation of Korea [2012R1A2A2A01013466] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Total internal reflection fluorescence microscopy (TIRFM) was investigated for enhanced detection sensitivity of cardiac troponin I (cTnI) on a gold nano-patterned protein chip. A 4 x 5 nanoarray patterning of a gold chip based on a single-molecule sandwich immunoassay was prepared with a spot diameter of 500 nm on glass substrates formed via electron-beam nanolithography. Quantitative trace analysis was possible as a function of increasing total internal reflection fluorescence intensity in the range of 84 aM-350 pM at prism-type and 35 aM-350 pM at objective-type TIRFM. Although using same gold nano-patterned cTnI chips, however, the objective-type TIRFM showed enhanced detection sensitivities 2.4 times better than the prism-type due to weaker gold quenching. This result is also about 7000 times lower in the detection limit compared with those obtained by conventional commercial sandwich enzyme-linked immunosorbent assay techniques. (C) 2012 Elsevier B.V. All rights reserved.
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