Journal
TALANTA
Volume 94, Issue -, Pages 263-270Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2012.03.037
Keywords
Multiwalled carbon nanotubes; Gold/platinum hybrid; Zinc oxide nanorods; Cholesterol oxidase; Biosensor
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Funding
- National Natural Science Foundation of China [21075100]
- Key Lab of Chongqing Modern Analytical Chemistry [201004]
- Doctor Foundation of Southwest University [SWUB2008048]
- Fundamental Research Funds for the Central Universities [XDJK2009C082, XDJK2012A004]
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A novel scheme for the fabrication of gold/platinum hybrid functionalized ZnO nanorods (Pt-Au@ZnONRs) and multiwalled carbon nanotubes (MWCNTs) modified electrode is presented and its application for cholesterol biosensor is investigated. Firstly, Pt-Au@ZnONRs was prepared by the method of chemical synthesis. Then, the Pt-Au@ZnONRs suspension was dropped on the MWCNTs modified glass carbon electrode, and followed with cholesterol oxidase (ChOx) immobilization by the adsorbing interaction between the nano-material and ChOx as well as the electrostatic interaction between ZnONRs and ChOx molecules. The combination of MWCNTs and Pt-Au@ZnONRs provided a favorable environment for ChOx and resulted in the enhanced analytical response of the biosensor. The resulted biosensor exhibited a linear response to cholesterol in the wide range of 0.1-759.3 mu M with a low detection limit of 0.03 mu M and a high sensitivity of 26.8 mu A mM(-1). The calculated apparent Michaelis constant K-M(app) was 1.84 mM, indicating a high affinity between ChOx and cholesterol. (C) 2012 Elsevier B.V. All rights reserved.
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