Journal
TALANTA
Volume 93, Issue -, Pages 147-152Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2012.01.065
Keywords
Human breast milk; Retinol; alpha-Tocopherol; LLE; Sample preparation; Monolithic column
Categories
Funding
- Grant Agency of Charles University in Prague (GAUK) [124809/2011, SVV-2012-265-002]
Ask authors/readers for more resources
The liposoluble vitamins (retinol and alpha-tocopherol) concentration in human breast milk is of a cardinal knowledge especially for nutrition of prematurely born. It enables the feeding optimization of these important micronutrients for preterm infants. The novel rapid liquid-liquid extraction procedure for human breast milk investigation was developed and validated according to FDA guidelines. The recovery of retinol was 82-90% measured at three concentration levels 1.0, 2.5 and 5.0 mu mol/L, for alpha-tocopherol 92-109% at concentration levels 2.5, 5.0 and 10.0 mu mol/L. The repeatability of extraction procedure expressed as relative standard deviation was 3.26% for retinol and 4.79% for alpha-tocopherol. Developed extraction procedure was applied on 120 human breast milk samples. The separation of vitamins was completed using advantages of a monolithic column which accomplished demands of acceleration made by modern bio-analytical HPLC methodology. The analytes of interest were detected by diode-array detector at wavelengths 325 nm for retinol and 290 nm for alpha-tocopherol. (C) 2012 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available