Journal
TALANTA
Volume 91, Issue -, Pages 52-59Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2012.01.009
Keywords
Vinyl-based monolithic column; Thiol-ene reaction; Click chemistry; n-Octadecanethiol; 3-Mercapto-1-propane-sulfonate
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Funding
- National Natural Science Foundation of China [20975032, 21075031]
- National Basic Research Program of China (973 Program) [2009CB421601]
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An n-octadecanethiol (C-18)/3-mercapto-1-propane-sulfonate (MPS) modified organic-inorganic hybrid silica monolithic column possessing vinyl ligands through thiol-ene click chemistry for capillary electrochromatography (CEC) is described. The proposed column is prepared via the sol-gel process by in situ co-condensation using vinyltrimethoxysilane (VTMS) and tetra-methoxysilane (TMOS) as precursors. Examination by SEM shows that the capillary has homogenous macroporous morphology and is well attached to the inner wall of the capillary. The obtained C-18-MPS-VTMS silica hybrid monolithic column demonstrated an enhanced hydrophilic property and could be applied as a reversed-phase stationary phase in CEC directly. Compared with unmodified VTMS silica hybrid monolithic column, stronger EOF was observed using this monolithic column. VTMS/TOMS ratios in the reaction mixture were varied and 1:3 was found to be optimum. Good separations of benzenes, aromatic amines, acids and peptides were achieved, the lowest plate height of similar to 3 mu m was obtained, the peak symmetry range from 0.98 to 1.29. The resulting C-18-MPS-VTMS silica hybrid monolithic column can be used in different separation modes, including reversed phase mode and ion exchange mode. (C) 2012 Published by Elsevier ay.
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