Journal
TALANTA
Volume 85, Issue 5, Pages 2576-2580Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2011.08.018
Keywords
Human chorionic gonadotropin (hCG); Label-free impedimetric immunosensor; Conducting polymer; Polypyrrole; Electrochemical impedance spectroscopy (EIS); Screen-printed electrode; DEP chip
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Funding
- Japan Society for the Promotion of Science (JSPS)
- Grants-in-Aid for Scientific Research [10F00057] Funding Source: KAKEN
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In this work, a sensitive label-free impedimetric hCG-immunosensor was constructed by using a commercial screen-printing carbon ink electrode (namely disposable electrochemical printed chip) as the basis. The carbon ink electrode of DEP chip is modified first by deposition of polypyrrole-pyrole-2-carboxylic acid copolymer and thence hCG antibody immobilization via the COOH groups of pyrrole-2-carboxylic acid, which can serve as a linker for covalent biomolecular immobilization. The experimental results exposed that the designed immunosensor is more sensitive than other previously reported immunosensors, in the case of detection limit and linear range for antigen detection. With optimal fabrication parameters, the detection limit for alpha-hCG was 2.3 pg/mL in 10 mM phosphate buffer saline (PBS) solution containing 1% bovine serum albumine (BSA). Moreover, the use of inexpensive DEP chip as a basis for these immunosensors will allow for simple instrumentation, disposable and portable at low cost. This work also demonstrates a new approach to develop a sensitive and label-free impedimetric immunosensor based on screen-printed electrode for applications in clinical diagnosis. (C) 2011 Elsevier B.V. All rights reserved.
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