Journal
TALANTA
Volume 85, Issue 1, Pages 183-196Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2011.03.048
Keywords
Pesticides; Solid phase extraction; Urine samples; Gas chromatography; Liquid chromatography; Mass spectrometry
Categories
Funding
- Consejeria de Economia, Innovacion y Ciencia de la Junta de Andalucia-FEDER [P05-FQM-0202]
- Spanish Ministry of Science and Innovation
Ask authors/readers for more resources
A new multiresidue method has been developed and validated for the simultaneous extraction of more than two hundred pesticides, including non-polar and polar pesticides (carbamates, organochlorine, organophosphorous, pyrethroids, herbicides and insecticides) in urine at trace levels by gas and ultra high pressure liquid chromatography coupled to ion trap and triple quadrupole mass spectrometry, respectively (GC-IT-MS/MS, UHPLC-QqQ-MS/MS). Non-polar and polar pesticides were simultaneously extracted from urine samples by a simple and fast solid phase extraction (SPE) procedure using C-18 cartridges as sorbent, and dichloromethane as elution solvent. Recovery was in the range of 60-120%. Precision values expressed as relative standard deviation (RSD) were lower than 25%. Identification and confirmation of the compounds were performed by the use of retention time windows, comparison of spectra (GC-amenable compounds) or the estimation of the ion ratio (LC-amenable compounds). For GC-amenable pesticides, limits of detection (LODs) ranged from 0.001 to 0.436 mu g L-1 and limits of quantification (LOQs) from 0.003 to 1.452 mu g L-1. For LC-amenable pesticides, LODs ranged from 0.003 to 1.048 mu g L-1 and LOQs ranged from 0.011 to 3.494 mu g L-1. Finally, the optimized method was applied to the analysis of fourteen real samples of infants from agricultural population. Some pesticides such as methoxyfenozide, tebufenozide, piperonyl butoxide and propoxur were found at concentrations ranged from 1.61 to 24.4 mu g L-1, whereas methiocarb sulfoxide was detected at trace levels in two samples. (C) 2011 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available