Journal
TALANTA
Volume 85, Issue 1, Pages 104-111Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2011.03.040
Keywords
Polyaniline; Multi-wall carbon nanotube; Biosensor; Platinum nanoparticles; Glucose oxidase; Composites
Categories
Funding
- National Natural Science Foundation of China [21075100]
- Ministry of Education of China [708073]
- Natural Science Foundation of Chongqing City [CSTC-2009BA1003]
- Southwest University, China
- High Technology Project Foundation of Southwest University, China [XSGX 02]
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A new glucose amperometric biosensor, based on electrodeposition of platinum nanoparticles onto the surface of multi-wall carbon nanotube (MWNT)-polyaniline (PANI) nanocomposites, and then immobilizing glucose oxidase (GOD) with covalent interaction and adsorption effect, was constructed in this paper. Firstly, the MWNT-PANI nanocomposites had been synthesized by in situ polymerization and were characterized through transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, and ultraviolet and visible (UV-vis) absorption spectra. The assembled process of the modified electrode was probed by scanning electron microscopy (SEM) and cyclic voltammetry (CV). Chronoamperometry was used to study the electrochemical performance of the resulting biosensor. The glucose biosensor exhibited a linear calibration curve over the range from 3.0 mu M to 8.2 mM, with a detection limit of 1.0 mu M and a high sensitivity of 16.1 mu A mM(-1). The biosensor also showed a short response time (within 5 s). Furthermore, the reproducibility, stability and interferences of the biosensor were also investigated. (C) 2011 Elsevier B.V. All rights reserved.
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