Journal
TALANTA
Volume 85, Issue 2, Pages 1174-1179Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2011.05.038
Keywords
Graphene; Alcohol dehydrogenase; NADH; Ethanol biosensor
Categories
Funding
- NSFC [20925517]
- STCSM [10XD1406000, 09JC1402600]
- Shanghai Leading Academic Discipline [B109]
- [SKLEAC201101]
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Characterization and application of graphene sheets modified glassy carbon electrodes (graphene/GC) have been presented for the electrochemical bio-sensing. A probe molecule, potassium ferricyanide is employed to study the electrochemical response at the graphene/GC electrode, which shows better electron transfer than graphite modified (graphite/GC) and bare glassy carbon (GC) electrodes. Based on the highly enhanced electrochemical activity of NADH, alcohol dehydrogenase (ADH) is immobilized on the graphene modified electrode and displays a more desirable analytical performance in the detection of ethanol, compared with graphite/GC or GC based bio-electrodes. It also exhibits good performance of ethanol detection in the real samples. From the results of electrochemical investigation, graphene sheets with a favorable electrochemical activity could be an advanced carbon electrode materials for the design of electrochemical sensors and biosensors. (C) 2011 Elsevier B.V. All rights reserved.
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