Journal
TALANTA
Volume 83, Issue 3, Pages 955-959Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2010.10.057
Keywords
Soft tissue injury; Liver injury; Abdominal trauma; Biosensor; Logic gate; Serum
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Funding
- Office of Naval Research [N00014-08-1-1202]
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Digital biosensor systems analyzing biomarkers characteristic of liver injury (LI), soft tissue injury (STI) and abdominal trauma (ABT) were developed and optimized for their performance in serum solutions spiked with injury biomarkers in order to mimic real medical samples. The systems produced 'Alert'-type optical output signals in the form of YES-NO separated by a threshold value. The new approach aims at the reliable detection of injury biomarkers for making autonomous decisions towards timely therapeutic interventions, particularly in conditions when a hospital treatment is not possible. The enzyme-catalyzed reactions performing Boolean AND/NAND logic operations in the presence of different combinations of the injury biomarkers allowed high-fidelity biosensing. Robustness of the systems was confirmed by their operation in serum solutions, representing the first example of chemically performed logic analysis of biological fluids and a step closer towards practical biomedical applications of enzyme-logic bioassays. (C) 2010 Elsevier B.V. All rights reserved.
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