Journal
TALANTA
Volume 82, Issue 5, Pages 1912-1918Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2010.08.009
Keywords
Capillary electrophoresis; Amino acid; Chiral separation; Cerebrospinal fluid; Naphthalene-2,3-dicarboxaldehyde; Light-emitting diode
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Funding
- National Science Council of Taiwan [NSC 96-2113-M-242 -003]
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We describe the stacking and separation of D- and L-aspartic acid (Asp) by capillary electrophoresis (CE) with light-emitting diode-induced fluorescence detection (LEDIF). In the presence of cyanide, D- and L-Asp were derivatized with naphthalene-2,3-dicarboxaldehyde (NDA) to form fluorescent derivatives prior to CE-LEDIF. The separation of NDA-derivatized D- and L-Asp was accomplished using a discontinuous system - buffer vials contained a solution of 0.6% poly(ethylene oxide) (PEO). 150 mM sodium dodecyl sulfate (SOS), and 60 mM hydroxypropyl-beta-cyclodextrin (Hp-beta-CD), while a capillary was filled with a solution of 150 mM SOS and 60 mM Hp-beta-CD. The role of PEO, Hp-beta-CD. and SOS is to act as a concentrating media, as a chiral selector, and as a pseudostationary phase, respectively. This discontinuous system could be employed for the stacking of 600 nL of NDA-derivatized D- and L-Asp without the loss of chiral resolution. The stacking mechanism is mainly based on the difference in viscosity between sample zone and PEO as well as SOS sweeping. The limits of detection at signal-to-noise of 3 for D- and L-Asp were down to 2.4 and 2.5 x 10(-10) M, respectively. Compared to normal sample injection volume (25 nL), this stacking approach provided a 100- and 110-fold improvement in the sensitivity of D- and L-Asp, respectively. This method was further applied for determining D- and L-Asp in cerebrospinal fluid, soymilk, and beer. (C) 2010 Elsevier B.V. All rights reserved.
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