Journal
TALANTA
Volume 80, Issue 3, Pages 1287-1291Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2009.09.026
Keywords
Hg2+; Nanogold-aptamer catalysis; HAuCl4; Ascorbic acid; Resonance scattering spectral assay
Categories
Funding
- Natural Science Foundation of Guangxi [0832260, 0991021Z]
- Research Funds of Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment [0701Z022, 0701k008]
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Single strand DNA (ssDNA) was used to modify nanogold to obtain a nanogold-aptamer resonance scattering(RS) probe (NGssDNA) for Hg2+, based on the formation of stable thymine-Hg2+-thymine (T-Hg2+-T) mismatches and aggregation of the released nanogold particles. After removing the aggregated particles by filtrate membrane, the excess NGssDNA in the filtration solution exhibit catalytic effect on the gold particle reaction between HAuCl4 and ascorbic acid (AA) that appear as RS peak at 596 nm. When Hg2+ concentration increased, the RS intensity at 596 nm decreased. The decreased intensity is linear to Hg2+ concentration in the range of 0.00008-0.888 ng/mL Hg2+, with detection limit of 0.000034 ng/mL The nanogold-aptamer catalytic RS assay was applied to determination of Hg2+ in water with satisfactory results. (C) 2009 Elsevier B.V. All rights reserved.
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