Journal
TALANTA
Volume 81, Issue 1-2, Pages 722-726Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2009.12.001
Keywords
Centrifugal microfluidics; Absorbance; Fluorescence; PAH; Solid phase extraction; In situ detection
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Funding
- National Science and Engineering Research Council (NSERC)
- Discovery Grant Program [RGPIN 1126]
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Great variations in pollutant concentrations are observed in the environment and pre-concentration is often required to detect trace contaminants in water samples. This paper presents a novel solid phase-extraction device integrated onto a centrifugal microfluidic platform for rapid on-site pre-concentration and screening of organic contaminants in aqueous samples. In-column fluorescence and absorbance measurements are obtained directly from an analyte trapped on the top of a solid phase extraction microcolumn. Results are presented for the representative fluorophore fluorescein and the polycyclic aromatic hydrocarbon anthracene. An absolute detection limit of 20 ng was obtained for anthracene using a simple light emitting diode for fluorescence excitation. One of the main advantages of this device is that only a simple motor is needed to induce liquid flow, making simultaneous on-site extraction and measurement of multiple samples easy while minimizing sample losses and contamination. (C) 2010 Elsevier B.V. All rights reserved.
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