Journal
TALANTA
Volume 82, Issue 3, Pages 957-961Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2010.05.070
Keywords
PEDOT:PSS; Acetycholinesterase; Mediator; Organophosphates
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This work describes the use of a PEDOT:PSS-based conductive polymer for designing AChE-based biosensors. The transducers were obtained directly by screen-printing a PEDOT:PSS suspension on the surface of thick film carbon electrodes. The obtained working electrodes showed a high conductivity when compared with electrodes modified with conventional mediators like cobalt phthalocyanine or tetracyanoquinodimethane. The PEDOT:PSS polymer was shown to be suitable for thiocholine oxidation, allowing the measurement of AChE activity at 100 mV vs Ag/AgCl. The high conductivity of PEDOT:PSS allowed the accurate detection of the organophosphate insecticide chlorpyrifos-oxon at concentrations as low as 4 x 10(-9) M, corresponding to an inhibition ratio of 5%. (C) 2010 Elsevier B.V. All rights reserved.
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