Journal
TALANTA
Volume 82, Issue 4, Pages 1349-1357Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2010.06.065
Keywords
Two-dimensional HPLC; Natural Products; Selectivity; Coffee
Categories
Funding
- UWS
- Deakin University
- UWS-Phenomenex
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Differences between alkyl, dipole-dipole, hydrogen bonding, and pi-pi selective surfaces represented by non-resonance and resonance pi-stationary phases have been assessed for the separation of 'Ristretto' cafe espresso by employing 2DHPLC techniques with C18 phase selectivity detection. Geometric approach to factor analysis (GAFA) was used to measure the detected peaks (N), spreading angle (beta), correlation, practical peak capacity (n(p))and percentage usage of the separations space, as an assessment of selectivity differences between regional quadrants of the two-dimensional separation plane. Although all tested systems were correlated to some degree to the C18 dimension, regional measurement of separation divergence revealed that performance of specific systems was better for certain sample components. The results illustrate that because of the complexity of the 'real' sample obtaining a truly orthogonal two-dimensional system for complex samples of natural origin may be practically impossible. (C) 2010 Elsevier B.V. All rights reserved.
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