Journal
TALANTA
Volume 81, Issue 4-5, Pages 1281-1287Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2010.02.021
Keywords
Hydrophilic interaction liquid; chromatography; Retention behaviour; Resolution; Mixture design; Desirability function; Glycerol; Urea
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A chemometric approach was used to study the retention behaviour of glycerol, urea and glycerol carbonate in hydrophilic interaction liquid chromatography (HILIC) First, a simplex method was developed to optimize the sensitivity of an evaporative light scattering detector A mixture design was then applied to model retention factors as a function of the mobile phase content in acetonitrile, water and methanol on three columns Atlantis HILIC Silica, ZIC-HILIC and Monochrom diol. Atlantis HILIC Silica exhibits predominantly hydrophobic interactions, while retention on the other two columns is mainly ruled by hydiophilic interactions. Finally, a desirability function is applied on the resolution factors. The use of this function enables the compositions of eluent phases to be determined in order to achieve separation between the three chemicals. Monochrom diol proved to be the most efficient column. (C) 2010 Elsevier B.V All rights reserved
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