Journal
TALANTA
Volume 79, Issue 3, Pages 836-844Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2009.05.010
Keywords
Atherosclerosis; Metabonomics; UFLC/MS-IT-TOF; Plasma; Urine; Rats
Categories
Funding
- National Basic Research Program of China [2005CB523301, 2006CB503902]
- ICST [2006DFB32460]
- State Ministry of Science & Technology of China [2006BAK02A12]
- National Natural Science Foundation of China [20675082, 90709004]
- Chinese Academy of Sciences [K2006A12-K2006A14]
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An ultra fast liquid chromatography coupled with IT-TOF mass spectrometry (UFLC/MS-IT-TOF) metabonomic approach was employed to study the plasma and urine metabolic profiling of atherosclerosis rats. Acquired data were subjected to principal component analysis (PCA) for differentiating the atherosclerosis and the control groups. Potential biomarkers were screened by using S-plot and were identified by the accurate mass and MSn fragments information obtained from UFLC/MS-IT-TOF analysis. 12 metabolites in rat plasma and 8 metabolites in urine were identified as potential biomarkers. Concentrations of leucine, phenylalanine, tryptophan, acetylcarnitine, butyrylcarnitine, propionylcarnitine and spermine in plasma and 3-O-methyl-dopa, ethyl, N2-acetyl-L-argininate, leucylproline, glucuronate, t6A N(6)-(N-threonylcarbonyl)-adenosine and methyl-hippuric acid in urine decreased in atherosclerosis rats. Ursodeoxycholic acid, chenodeoxycholic acid, LPC (06:0), LPC (C18:0) and LPC (C18:1) in plasma and hippuric acid in urine were in higher levels in atherosclerosis rats. The alterated metabolites demonstrated abnormal metabolism of phenylalanine, tryptophan, bile acids and amino acids. This research proved that metabonomics is a promising tool for disease research. (C) 2009 Elsevier B.V. All rights reserved.
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