Journal
TALANTA
Volume 80, Issue 2, Pages 651-655Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2009.07.043
Keywords
Immunoaffinity extraction; High-performance liquid chromatography (HPLC); Tandem mass spectrometry (MS/MS); Indole-3-acetic acid (IAA); Auxin conjugates; Helleborus niger L.
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Funding
- Ministry of Education, Youth and Sports of the Czech Republic (MSM) [6198959216]
- Grant Agency of the Academy of Sciences CR (GACR) [522/08/H003, KAN200380801]
- Croatian Ministry of Science, Education and Sports [098-0982913-2829]
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An analytical protocol for the isolation and quantification of indole-3-acetic acid (IAA) and its amino acid conjugates was developed. IAA is an important phytohormone and formation of its conjugates plays a crucial role in regulating IAA levels in plants. The developed protocol combines a highly specific immunoaffinity extraction with a sensitive and selective LC-MS/MS analysis. By using internal standards for each of the studied compounds, IAA and seven amino acid conjugates were analyzed in quantities of fresh plant material as low as 30 mg. In seeds of Helleborus niger, physiological levels of these compounds were found to range from 7.5 nmol g(-1) fresh weight (IAA) to 0.44 pmol g(-1) fresh weight (conjugate with Ala). To our knowledge, the identification of IAA conjugates with Gly, Phe and Val from higher plants is reported here for the first time. (C) 2009 Elsevier B.V. All rights reserved.
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