Journal
TALANTA
Volume 78, Issue 1, Pages 284-289Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2008.11.018
Keywords
ZnO nanostructures; Flower-shaped nanostructures; Cholesterol oxidase; Cholesterol biosensor
Categories
Funding
- Korea Research Foundation [KRF-2005-005-J07502]
- Korea Science and Engineering Foundation [R01-2006-000-11306-0]
- Korean Government (MEST)
- National Research Foundation of Korea [과C6B1912, 2005-005-J07502, R01-2006-000-11306-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
This paper reports the fabrication of highly-sensitive cholesterol biosensor based on cholesterol oxidase (ChOx) immobilization on well-crystallized flower-shaped ZnO structures composed of perfectly hexagonal-shaped ZnO nanorods grown by low-temperature simple solution process. The fabricated cholesterol biosensors reported a very high and reproducible sensitivity of 61.7 mu A mu M-1 cm(-2) with a response time less than 5s and detection limit(based on S/N ratio) of 0.012 mu M. The biosensor exhibited a linear dynamic range from 1.0-15 mu M and correlation coefficient of R=0.9979. A lower value of apparent Michaelis-Menten constant (K-m(aPP)), of 2.57 mM,exhibited a high affinity between the cholesterol and ChOx immobilized on flower-shaped ZnO structures. Moreover, the effect of pH on ChOx activity on the ZnO modified electrode has also been studied in the range of 5.0-9.0 which exhibited a best enzymatic activity at the pH range of 6.8-7.6. To the best of our knowledge. this is the first report in which such a very high-sensitivity and low detection limit has been achieved for the cholesterol biosensor by using ZnO nanostructures modified electrodes. (c) 2008 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available