Journal
TALANTA
Volume 78, Issue 1, Pages 310-314Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2008.11.015
Keywords
Honey; 5-Hydroxymethyl-2-furaldehyde (HMF); Furfural; 2-Furaldehyde; 3-Furaldehyde; 2-Furoic acid; 3-Furoic acid
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In this study 5-hydroxymethyl-2-furaldehyde (HMF), 2-furaldehyde, 3-furaldehyde, 2-furoic acid and 3-furoic acid are contemporarily determined in honey using a swift and direct RP-HPLC approach. The validation protocol was performed in terms of detection and quantification limits, precision (by repeatability and reproducibility), linearity and accuracy (by recovery tests); the acceptability of the precision and accuracy results was positively verified using Horwitz's model and AOAC guidelines. respectively. The method was tested on 18 honey samples of different ages, and botanical and geographical origin. HMF and 2-furaldehyde correlated highly with the age of the samples, whereas no correlation was observed with regards to 2-furaldehyde and 2-furoic acid. Hypotheses relating to the formation of minority furanic compounds are also proposed. (c) 2008 Elsevier B.V. All rights reserved.
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