Journal
TALANTA
Volume 78, Issue 1, Pages 40-48Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2008.10.037
Keywords
Triterpenic compounds; LC-MS-MS; Multiple reaction monitoring (MRM); Natural products; Microwave-assisted extraction
Categories
Funding
- Spain's Ministry of Science and Innovation [CTQ2006-01614]
Ask authors/readers for more resources
A method for fast and selective determination of the main triterpenic compounds present in olive leaves oleanolic, ursolic and maslinic acids as triterpenic acids and, uvaol and erythrodiol as triterpenic dialcohols - is reported here. Quantitative isolation of the analytes has been accomplished in 5 min by microwave assistance using ethanol as extractant. Due to the medium polarity of triterpenic acids and dialcohols, different ethanol-water ratios were tested in order to select the optimum extractant composition for their solubilisation. Microwave assistance provided a significant shortening of the leaching time as compared to conventional procedures by maceration, which usually requires at least 5 h. After extraction, determination was carried out by liquid chromatography-tandem mass spectrometry (LC-MS-MS) with a triple quadrupole (qQq) mass detector without any clean-up step prior to chromatographic analysis. Highly selective identification of triterpenes was confirmed by multiple reaction monitoring (MRM) using the most representative transitions from the precursor ion to the different product ions, while the most sensitive transitions were used for MS-MS quantitation. Total analysis performed in 25 min enables the characterization of a fraction with particular interest in the pharmacological area. (C) 2008 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available