Journal
TALANTA
Volume 79, Issue 3, Pages 775-779Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2009.05.001
Keywords
Mercury ion; Photoinduced charge transfer; Fluorescence quenching; M-DNA
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Funding
- National Natural Science Foundation [50503006, 20575011, 20735002]
- Science foundation of Fujian Province [2008J0149]
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A novel and simple oligodeoxyribonucleotide-based sensor with single fluorophore-labeled for mercury ion sensing was reported. An oligodeoxyribonucleotide poly(dT) was labeled with fluorescein as donor. Based on the specific binding of Hg(II) to T-T mismatch base pairs, the formation of pi-stacked [T-Hg(II)-T] with sandwich structure on the addition of Hg(II) ions facilitates the electron transfer via photoinduced charge transfer (PCT), which creates an additional nonradiative decay channel for excited fluorophore and triggers the fluorescence to be quenched. The pi-stacked [T-Hg(II)-T] functioned not only as mercury ion recognition but also as an electron acceptor to quench the donor. A linear relationship was observed over the range of 0-1.0 mu M with the detection limit of 20 nM for mercury ions. The fluorescence quenching phenomenon and quenching mechanism, reliability and selectivity of the system were investigated in detail. (C) 2009 Elsevier B.V. All rights reserved.
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