Journal
TALANTA
Volume 79, Issue 2, Pages 289-294Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2009.03.046
Keywords
Impedance spectroscopy; Amperometry; Cytochrome c-based biosensor; Antioxidant capacity; Screen-printed gold electrodes
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Funding
- European Commission [FOOD-CT-2005-023044]
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This paper describes the characterization and optimization of an amperometric cytochrome c(cyt c)-based sensor for the determination of the antioxidant capacity of pure substances and natural samples. The cyt c and the xanthine oxidase (XOD) enzyme were co-immobilized on the electrode using the combination of several long-chain thiols. The self-assembled monolayer (SAM) was optimized in terms of composition and ratio between thiols. The immobilization protocol for both cyt c and XOD and the SAM formation time were evaluated through electrochemical methods, such as cyclic voltammetry (CV), square wave voltammetry (SWV), chronoamperometry (CA) and impedance spectroscopy (IS). Finally, the biosensor was applied to the determination of the antioxidant capacity Of pure alliin and two compounds extracted from garlic bulbs. (C) 2009 Elsevier B.V. All rights reserved.
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